| Assay Method Information | |
| | Inhibition Activity of Compounds of the Present Invention on TYK2 JH2 Domain |
| Description: | Compound of the present invention formulated into different concentrations (series 3 times dilutions, started from 60 μM, 11 concentrations in total) was added to 384-well plate, followed by 5 μL of enzyme (i.e., TYK2 JH2 domain), Tb antibody (Cisbio) and tracer. The mixture was incubated at 25° C. for 60 min and left at 4° C. overnight. Based on the fluorescence signals of the reaction system at 665 nm and 615 nm read using Envision in FRET mode, inhibition rate was calculated with the following formula:%inhibition=1-[Ratiocmpd-Ratio_lowRatio_High-Ratio_Low]*100%wherein: Ratio meant the ratio of fluorescence signals (channel 665/channel 615); High meant high control, i.e., the fluorescence signal of the wells detected by the method which was operated similarly but no compound of the present invention was added; Low meant Low control, i.e., the fluorescence signal of the wells detected by the method which was operated similarly but neither enzyme nor compound of the present invention was added. |
| Affinity data for this assay | |
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