| Assay Method Information | |
| | Biological Assay |
| Description: | The following solutions were prepared for each 1×384 plate:Solution A: 2 mL Optimem with one of the following stimuli:60 μL of 10 mM 2′3′cGAMP→150 μM stockSolution B: 2 mL Optimem with 60 μL Lipofectamine 2000→Incubate 5 min at RT2 mL of solution A and 2 ml Solution B was mixed and incubated for 20 min at room temperature (RT). 20 μL of transfection solution (A+B) was added on top of the plated cells, with a final 2′3′cGAMP concentration of 15 μM. The plates were then centrifuged immediately at 340 g for 1 minute, after which they were incubated at 37° C., 5% CO2, >98% humidity for 24 h. Luciferase reporter activity was then measured. EC50 values were calculated by using standard methods known in the art. |
| Affinity data for this assay | |
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