| Assay Method Information | |
| | BTK(h) Study |
| Description: | Compounds of Formula Ib, Formula II, and Formula III were tested against BTK(h) as described herein. All compounds tested were prepared to a working stock of 50× final assay concentration in 100% DMSO. Where appropriate, more concentrated stocks were diluted manually to 50× using 100% DMSO. Compounds, which were powders, were reconstituted to a 10 mM stock in 100% DMSO before further dilution to 50×. Assay Procedure: The required volume of the 50× stock of test compound was added to the assay well before a reaction mix containing the enzyme and substrate was added. The reaction was initiated by the addition of ATP at the selected concentration. There was no pre-incubation of the compound with the enzyme/substrate mix prior to ATP addition. |
| Affinity data for this assay | |
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