| Assay Method Information | |
| | SARS-CoV-2 Immunofluorescence Assay 1 |
| Description: | Vero E6 cells derived from BCRC #60476 (Bioresource Collection and Research Center, Hsinchu, Taiwan) were treated with each compound at an indicated concentration for 1 hour at 37 C. The cells were adsorbed with SARS-CoV-2 viruses (TCDC #4, National Taiwan University, Taipei, Taiwan, ROC) at multiplicity of infection ( MOI ) of 0.01 for 1 hour at 37 C. After virus adsorption, the cells were washed with phosphate-buffered saline ( PBS ). A fresh medium containing the compound was added at an indicated concentration. The resultant mixture was incubated for 2 days. The cells were fixed with 4% paraformaldehyde and permeabilized with a 0.5% Triton X-100 detergent solution (Thermo Fisher Scientific, Waltham, MA). Subsequently, they were stained with an anti-SARS-CoV-2 N protein antibody and anti-human IgG-488 (in green). The nuclei of the cells were counter stained with 4′,6-diamidino-2-phenylindole ( DAPI , Thermo Fisher Scientific, MA, USA). The N protein expression was measured using a high-content image analysis system (Molecular Devices, San Jose, CA). The cell viability was determined by Cell Counting Kit-8 (Sigma-Aldrich, St. Louis, MO) EC50 and CC50 values were calculated by Prism software. |
| Affinity data for this assay | |
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