| Assay Method Information | |
| | Enzyme Activity Assay |
| Description: | Human OGA (recombinant hOGA protein, advanced Protein Technologies corp., Korea) enzyme reaction was performed in a reaction solution containing 25 mM Tris/HCl and 0.1 mg/ml bovine serum albumin (pH 7.5) using 2 mM 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (69585; Sigma) dissolved in DMSO as a substrate. The amount of the human OGA enzyme used in the reaction was 8 ng/well. Various amounts of the compounds of Examples 1 to 3 were added to the enzyme before the reaction started. The reaction was performed in a 384-well plate at 37° C. for 20 minutes, and then started by adding the substrate. An increase in fluorescence was measured using a SAFIRE (Tecan, Switzerland) fluorometer and detected at excitation and emission wavelengths set at 360 nm and 460 nm, respectively. |
| Affinity data for this assay | |
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