| Assay Method Information | |
| | Inhibitory Effects of the Compounds Disclosed Herein on ATPase Activity of Myosin |
| Description: | Myocardial actin (1.61 μM) and myosin motor protein S 1 fragment (0.07 μM) were mixed with different concentrations of small-molecule compounds (initial concentration of 100 μM, serially diluted 3-fold to 9 concentrations), and the plate was incubated at 37° C. for 1 h. Then 120 μM ATP was added and the plate was incubated at 37° C. for 2 h. Finally, the assay solution in the CytoPhos phosphate assay biochem kit was added to each well (70 μL/well), and the plate was incubated at room temperature for 10 min. The OD readings at the wavelength of 650 nM were taken on a microplate reader. The Pi amount was calculated according to the standard curve. The data were processed using GraphPad software. An inhibition curve was plotted according to the compound concentrations and the corresponding inhibition rates, and the concentration at which the inhibition rate was 50%, i.e., the IC50 value, was calculated. |
| Affinity data for this assay | |
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