| Assay Method Information | |
| | HCV NS5B Pol assay ([32P]-CTP]) |
| Description: | Compounds were assayed for inhibition of NS5B-821 from HCV GT-1b Con-1. Reactions included purified recombinant enzyme, 1 u/L negative-strand HCV IRES RNA template, and 1 μM NTP substrates including either [32P]-CTP. Assay plates were incubated at 27° C. for 1 hour before quench. [32P] incorporation into macromolecular product was assessed by filter binding. |
| Affinity data for this assay | |
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