| Assay Method Information | |
| | Solid Phase Integrin αvβ1 Binding Assay |
| Description: | Microplates were coated with recombinant human integrin αvβ1 (2 ug/ml) in PBS (100 ul/well 4° C., overnight). The coating solution was removed, washed with PBS. Plate was blocked with 200 ul/well of Block Buffer (2% BSA in PBS) at 37° C. for 1 h. Dilutions of testing compounds and recombinant fibronectin (2 ug/ml) in binding and washing buffer (50 mM Tris-HCl, ph7.5; 0.1% BSA, 1 mM MnCl2; NaCl 150 mM; 0.02% Tween-20; 1 mM CaCl2); 1 mM MgCl2) were added. The plate was incubated for 2 hours at 25° C., washed, and incubated for 1 hour with Biotin-Anti-fibronectin. Bound antibody was detected by peroxidase-conjugated streptavidin. |
| Affinity data for this assay | |
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