| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | Freshly prepared Zn2-NDM-1 (50 nM) or Zn2-VIM-2 were first incubated with various concentrations of Bi(III) compounds for 1 h at 25° C. The assay was performed in a 1 cm quartz cuvette using the kinetic mode of Varian Cary 50 UV-visible spectrophotometer at 25° C. The final assay buffer contains 50 mM HEPES/Na pH 7.0, 100 mM NaCl and 100 μM MER. The decrease in absorbance at 300 nm due to ring-opening of MER was monitored every second for a duration of 10 mins until the reaction was completed. The initial rate were extracted and calculated from each reaction curves. |
| Affinity data for this assay | |
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