| Assay Method Information | |
| | Inhibition Assay |
| Description: | Table 1 shows the IC50 of curcumin, compound 1, and compound 2, compared to a standard Zn++ binding & MMPI (matrix metalloproteinase inhibitor), 1,10-O-phenanthroline (o-phen), against purified human PMN MMP-8 (from EMD biologics, Inc., Gibbstown, N.J.) using a synthetic octapeptide containing the collagenase-susceptible glycine-isoleucine peptide bond and measuring the tripeptide breakdown products by HPLC (Waters Alliance 2695 System with a reverse phase C-18 column). Compound 1 was an excellent MMPI with an IC50 equivalent to that of 1,10-O-phenanthroline, while compound 2, which lacked substituents on the aryl moieties, did not show a dose response. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |