| Assay Method Information | |
| | USP7 assay |
| Description: | Dilute the compounds to 400 x of the final desired highest inhibitor concentration in reaction by 100% DMSO. For all compounds, transfer the compounds to one well in a 384-well Echo plate and serially dilute the compound by 3-fold dilution of 100% DMSO in the next well and so forth for a total of 10 concentrations by Precision. Add 30μL of 100% DMSO to two empty wells for no compound control and no enzyme control in the same 384-well Echo plate. Make the plate as a source plate. |
| Affinity data for this assay | |
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