| Assay Method Information | |
| | Activity Assays |
| Description: | The ability of the sphingo sine analogs presented herein to kill Pseudomonas aeruginosa in vitro, and based on the data shown below, concluded that the SPH analogs presented herein are highly efficient in killing bacteria. Compounds 791598 and 791577 were used as activity assay control references. To determine whether the sphingosine analogs presented herein can be metabolized by ceramide synthases (CerS natural sphingosine is a substrate for CerS), their N-acylation using 14C labeled CoA as a substrate was examined. The results confirmed that neither 791995 nor 791996 could be N-acylated, demonstrating that these SPH analogs are not substrates for CerS, and indicating that there is no concern that N-acylation might be an off-target effect (raw data and results not shown). |
| Affinity data for this assay | |
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