| Assay Method Information | |
| | Nrf2-Keapl Biochemical Assay Protocol |
| Description: | In a first step, the Keapl /compound complex was prepared by addition of 100 nL of the compound by ECHO 555, followed by 5 pL aliquots of 2x Keapl. This mixture was incubated for 15 minutes at room temperature. In a second step, the Keapl/Nrf2 complex was prepared by adding 5 p L aliquots of 2x Nrf2 to the same mixture and incubating again for 15 minutes at room temperature. 5 pL of anti HIS-Tb was then added, followed by the addition of 5 pL of 4x streptavidin-XL665. After incubation at 4°C for 2hours, TR-FRET measurements were made on Pherastar FS (optic module HTRF 337/620/665nM). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |