| Assay Method Information | |
| | Homogenouse Time-Resolved Fluorescence (HTRF) Binding Assay |
| Description: | Experimental Procedure1. The compound was formulated to 10 concentrations with a three-fold concentration gradient with 100% DMSO.2. The solution of the compound in DMSO was added to Dilute Buffer, and mixed evenly and then transferred to a 96-well plate.3. PD-L1 was diluted with Dilute Buffer and added to the 96-well plate above.4. PD-1 was diluted with Dilute Buffer, then added to the 96-well plate above and incubated at room temperature for 30 minutes.5. One portion of anti-tag1-Eu and one portion of anti-tag2-XL665 were added to Detection Buffer, mixed evenly and transferred to the 96-well plate above.6. The mixed solution in the 96-well plate were incubated at room temperature for 1 to 24 hours.7. HTRF values were read by Envision. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |