Assay Method Information | |
| Measurement of DHODH Inhibitory Enzyme Activity (In Vitro Assays) |
Description: | The DHODH activity assay is a coupled enzyme assay in which oxidation of DHO and subsequent reduction of ubiquinone are stoichiometrically equivalent to the reduction of DCIP (2,6-dichlorophenol). The reduction of DCIP is accompanied by a loss of absorbance at 610 nm. |
Affinity data for this assay | |
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