| Assay Method Information | |
| | Homogeneous Time-Resolved Fluorescence (HTRF) Binding Assay |
| Description: | 1. The compound was formulated to 10 concentrations with a 3-fold gradient with 100% DMSO.2. The solution of the compound in DMSO was added to Dilute Buffer, mixed thoroughly, then transferred to a 96-well plate.3. PD-L1 was diluted with Dilute Buffer, then added to the above 96-well plate.4. PD-1 was diluted with Dilute Buffer and added to the above 96-well plate, which was then incubated at room temperature for 30 minutes.5. A portion of anti-tag1-Eu and a portion of anti-tag2-XL665 were added to Detection Buffer, mixed thoroughly and transferred to the above 96-well plate.6. The mixture in the above 96-well plate was incubated at room temperature for 1 to 24 hours.7. HTRF values were read with Envision. |
| Affinity data for this assay | |
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