Cell Reactant:

Syringe Reactant:

Meas. Tech.:

ITC

Entry Date.:

2000-12-11

ΔG°:

-6.63±0.03 (kcal/mole)

pH:

7.5000±n/a

Log₁₀Kb:

3.5

Temperature:

293.1500±n/a (K)

ΔH° :

-10.25±n/a (kJ/mole)

ΔH_obs :

-10.25±n/a (kJ/mole)

ΔS° :

-0.01±0 (kJ/mole-K)

Citation

 Taquet, A; Labarbe, R; Houssier, C Calorimetric investigation of ethidium and netropsin binding to chicken erythrocyte chromatin. Biochemistry 37:9119-26 (1998) [PubMed]  Article

More Info.:

Get all data from this article,  ITC RUN data,  Solution Info,  Instrument Info

Source:

Obtained from freshly killed chicken

Prep. Method:

nuclei were lysed and dialyzed against buffer, soluble chromatin were collected by centrifugation.H1 and H5 histones were depleted by adding NaCl 4M to chromatin solution up to 650 mM

Name:

H1/H5-depleted chromatin

Synonyms:

n/a

Type:

Other

Mol. Mass.:

n/a

Description:

H1/H5 were depleted from chicken erythrocyte chromatin

Components:

This complex has 0 components.

Name:

Histone deacetylase 1

Synonyms:

HD1 | Histone deacetylase 1 (HDAC1) | Human HDAC1 | HDAC1_HUMAN | HDAC1 | RPD3L1 | Cereblon/Histone deacetylase 1

Type:

Enzyme

Mol. Mass.:

55090.27

Organism:

Human

Description:

Q13547

Residue:

482

Sequence:

MAQTQGTRRKVCYYYDGDVGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKANAEEMTKYHSDDYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVASAVKLNKQQTDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNYPLRDGIDDESYEAIFKPVMSKVMEMFQPSAVVLQCGSDSLSGDRLGCFNLTIKGHAKCVEFVKSFNLPMLMLGGGGYTIRNVARCWTYETAVALDTEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA

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Blast E-value cutoff:

Name:

Histone deacetylase 4

Synonyms:

HD4 | Histone acetylase 4(HDAC4) | Human HDAC4 | HDAC4_HUMAN | HDAC4 | KIAA0288 | Cereblon/Histone deacetylase 4

Type:

Enzyme

Mol. Mass.:

119049.39

Organism:

Human

Description:

P56524

Residue:

1084

Sequence:

MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLGLPATGPSAGTAGQQDAERLTLPALQQRLSLFPGTHLTPYLSTSPLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQQLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEAHAQAGVQVKQEPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFPVSVQEPPTKPRFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQLMGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL

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Blast E-value cutoff:

Source:

Serva(heidelberg, Germany)

Name:

BDBM17

Synonyms:

4-(2-carbamimidamidoacetamido)-N-{5-[(2-carbamimidoylethyl)carbamoyl]-1-methyl-1H-pyrrol-3-yl}-1-methyl-1H-pyrrole-2-carboxamide | netropsin

Type:

Small organic molecule

Emp. Form.:

TBD

Mol. Mass.:

TBD

SMILES:

TBD

Structure:

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Similarity to this molecule at least: