Reaction Details  Report a problem with these data
Cell Reactant:
BirA Bifunctional Protein
Syringe Reactant:
BDBM12
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2007-12-19
ΔG°:
-9.89±0.04 (kcal/mole)
pH:
7.5000±0.2000
Log10Kb:
6.2
Temperature:
293.1500±0.1000 (K)
ΔHobs :
-19.2±0.1 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.03±0 (kJ/mole-K)
Citation
 Brown, PH; Beckett, D Use of binding enthalpy to drive an allosteric transition. Biochemistry 44:3112-21 (2005) [PubMed]  Article
Cell React
Source:
The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac
Purity:
>95%
Prep. Method:
The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps.
Name:
Bifunctional ligase/repressor BirA
Synonyms:
Biotin--[acetyl-CoA-carboxylase] synthetase | Biotin protein ligase (BPL) | Biotin operon repressor | BIRA_ECOLI | birA | bioR | dhbB | BirA Bifunctional Protein
Type:
Repressor protein
Mol. Mass.:
35313.58
Organism:
Escherichia coli (strain K12)
Description:
n/a
Residue:
321
Sequence:
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
  
Syringe React
Source:
Sigma-Aldrich
Name:
BDBM12
Synonyms:
cid_171548 | cid_253 | 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin
Type:
Enzyme co-factor
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: