Cell Reactant:

Syringe Reactant:

Meas. Tech.:

Isothermal Titration Calorimetry

Entry Date.:

2006-11-07

ΔG°:

-9.8±n/a (kcal/mole)

pH:

7.0000±n/a

Log₁₀Kb:

5.8

Temperature:

298.1500±n/a (K)

ΔH_obs :

-18±0.1 (kJ/mole)

Corrected for ΔH_ioniz:

not known

ΔC_p :

-0.21±n/a (kJ/mole)

Stoich. Param.:

1.0000

ΔS° :

-0.03±n/a (kJ/mole-K)

Citation

 Hoffman, JM; Grunau, A; Smith, AM; Paine, MJ; Rooney, CS; Ladbury, JE; Fisher, TE; Gutierrez, A; Wai, JS; Thomas, CM; Bamberger, DL; Barnes, JL; Williams, TM; Jones, JH Global effects of the energetics of coenzyme binding: NADPH controls the protein interaction properties of human cytochrome P450 reductase. Biochemistry 45:1421-34 (2006) [PubMed]  Article

More Info.:

Get all data from this article,  ITC RUN data,  Solution Info,  Data Fit Method,  Instrument Info

Source:

Human fibroblast CPR (lacking the N-terminal membrane-anchoring region) and the functional FAD-binding domain were expressed in Escherichia coli BL21 (DE3).

Prep. Method:

The recombinant His-tagged proteins were purified to homogeneity by nickel-agarose chromatography. The notable exception is the omission of the 2,5-ADP affinity resin step to avoid the unusual biphasic binding isotherms during ITC experiment.

Name:

NADPH--cytochrome P450 reductase

Synonyms:

NADPH--cytochrome P450 reductase | P450R | NCPR_HUMAN | POR | CYPOR | Cytochrome P450 Reductase (CPR)

Type:

Enzyme

Mol. Mass.:

76675.22

Organism:

Human

Description:

P16435

Residue:

677

Sequence:

MGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPAGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS

Blast this sequence in BindingDB or PDB

  
Blast E-value cutoff:

Source:

Sigma-Aldrich

Name:

BDBM11941

Synonyms:

{[(2R,3R,4R,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxy-4-(phosphonooxy)oxolan-2-yl]methoxy}phosphonic acid | ADENOSINE-2 -5 -DIPHOSPHATE | 2,5-ADP

Type:

Nucleoside or nucleotide

Emp. Form.:

TBD

Mol. Mass.:

TBD

SMILES:

TBD

Structure:

Search PDB for entries with ligand similarity:

Similarity to this molecule at least: