Cell Reactant:

Syringe Reactant:

Meas. Tech.:

ITC

Entry Date.:

2000-12-04

ΔG°:

-31.9200±2.5200 (kJ/mole)

pH:

8.5000±n/a

Log₁₀Kb:

4.9

Temperature:

298.1500±n/a (K)

ΔH° :

-8.4000±n/a (kJ/mole)

ΔH_obs :

-8.4000±n/a (kJ/mole)

ΔS° :

0.0756±0.2940 (kJ/mole-K)

Comments:

binding is very tight, limiting the accuracy of calorimetric measurement of the binding constant to approximately 25%

Citation

 Robinson, CR; Liu, Y; Thomson, JA; Sturtevant, JM; Sligar, SG Energetics of heme binding to native and denatured states of cytochrome b562. Biochemistry 36:16141-6 (1997) [PubMed]  Article

More Info.:

Get all data from this article,  ITC RUN data,  Solution Info,  Instrument Info

Source:

E.coli

Prep. Method:

Thermal denaturation

Name:

apocytochrome b562

Synonyms:

denatured apocytochrome b562 | apocytochrome b562(denatured) | C562_ECOLX | cybC

Type:

Protein

Mol. Mass.:

14060.82

Organism:

Escherichia coli

Description:

P0ABE7

Residue:

128

Sequence:

MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR

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Blast E-value cutoff:

Source:

Sigma Chemical Co.

Prep. Method:

dissolved in NaOH and diluted with buffer

Name:

BDBM20

Synonyms:

3-[5-(2-carboxyethyl)-15,20-diethenyl-22-hydroxy-4,10,14,19,24-pentamethyl-21,23,24,25-tetraaza-22-ferrahexacyclo[9.9.3.1^{3,6}.1^{13,16}.0^{8,23}.0^{18,21}]pentacosa-1(20),3,5,8,10,13,15,18-octaen-9-yl]propanoic acid | Hematin(iron protoporphyrin IX)

Type:

Porphyrin

Emp. Form.:

TBD

Mol. Mass.:

TBD

SMILES:

TBD

Structure:

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