BDBM50240826 AZD-1775::MK-1775::US11124518, Example AZD1775::US11248006, Compound AZD1775::US11479555, Example AZD1775::US12220415, Compound AZD1775::US20240182482, Compound MK-1775
SMILES CC(C)(c1cccc(n1)N2c3c(cnc(n3)Nc4ccc(cc4)N5CCN(CC5)C)C(=O)N2CC=C)O
InChI Key InChIKey=BKWJAKQVGHWELA-UHFFFAOYSA-N
Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB
Found 35 hits for monomerid = 50240826
Affinity DataIC50: 268nMAssay Description:Inhibition of PLK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 8.70nMAssay Description:In the present disclosure, Wee1 enzyme catalysis assay was carried out using the ATP-Glo Max kinase luminescence detection kit (Promega). Kinase acti...More data for this Ligand-Target Pair
Affinity DataIC50: 1.5nMAssay Description:In determination of the Weel kinase activity, a synthetic peptide, Poly(Lys,Tyr) Hydrobromide (Lys:Tyr (4:1)) bought from Sigma was used as the subst...More data for this Ligand-Target Pair
Affinity DataIC50: 3.90nMAssay Description:Displacement of Alexa Fluor labelled kinase tracer178 from WEE1 (unknown origin) incubated for 60 mins by FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.90E+4nMAssay Description:Inhibition of CYP3A4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 15nMAssay Description:Inhibition of human PLK1 in the presence of ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Inhibition of human Wee1 in the presence of ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 19nMAssay Description:Inhibition of WEE1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 5.20nMAssay Description:Inhibition of WEE1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Wee 1 kinase was determined by using Flurorescence Resonance Energy Transfer (FRET) assay. In 384-well plates, Wee1 kinase (2 nM final concentration)...More data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of recombinant human Wee1 (214 to end residues) incubated for 1 hr in presence of tracer 178 by fluorescence based assayMore data for this Ligand-Target Pair
Affinity DataIC50: 124nMAssay Description:Inhibition of human N-terminal His6-tagged recombinant full-length PLK1 expressed in baculovirus infected Sf9 cells incubated for 15 mins followed by...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of human full length GST-tagged WEE1 expressed in insect cells incubated for 15 mins followed by ATP addition by ADP Glo luminescent assayMore data for this Ligand-Target Pair
Affinity DataIC50: 128nMAssay Description:Inhibition of WEE1 in human U2OS cells incubated for 1 hr by mesoscale discovery assayMore data for this Ligand-Target Pair
Affinity DataIC50: 5.10nMAssay Description:Inhibition of recombinant human Wee1 incubated for 30 mins by liquid scintillation counter methodMore data for this Ligand-Target Pair
Affinity DataIC50: 0.560nMAssay Description:Inhibition of Wee1 (unknown origin) incubated for 60 mins by LanthaScreen Eu-Kinase binding assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.38nMAssay Description:After the serially diluted compound and an enzyme were mixed, the mixture was incubated at room temperature (25° C.) for 15 min, and then centrifuged...More data for this Ligand-Target Pair
Affinity DataIC50: 5.10nMAssay Description:A LanthaScreen kinase activity assay was conducted to assess WEE1 kinase inhibitory activity for compounds of this disclosure. LanthaScreen Eu time-r...More data for this Ligand-Target Pair
TargetMembrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase(Human)
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Affinity DataIC50: 1.34E+3nMAssay Description:Inhibition of N-[3',6'-dihydroxy-3-oxo-3H-spiro(2-benzofuran-1,9'-xanthen)-5-yl]-N'-[2-(4-{4-[N-(2-chloro-6-methylphenyl)-5-carboxamido]-thiazol-2-yl...More data for this Ligand-Target Pair
Affinity DataKd: 27nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged Wee2 kinase domain (202 to 492 residues) expressed in Escherichia coli BL21 (DE3) by iso...More data for this Ligand-Target Pair
Affinity DataKd: 110nMAssay Description:Binding affinity to human JAK2 JH1 domain assessed as dissociation constant by quantitative real-time PCR methodMore data for this Ligand-Target Pair
Affinity DataKd: 3nMAssay Description:Binding affinity to human PLK1 assessed as dissociation constant by quantitative real-time PCR methodMore data for this Ligand-Target Pair
Affinity DataKd: 3.90nMAssay Description:Binding affinity to recombinant human full-length N-terminal His8-tagged Wee2 (1 to 567 residues) expressed in human Expi293F cells assessed as desso...More data for this Ligand-Target Pair
Affinity DataKd: 26nMAssay Description:Binding affinity to human JAK3 JH1 domain assessed as dissociation constant by quantitative real-time PCR methodMore data for this Ligand-Target Pair
Affinity DataKd: 3.20nMAssay Description:Binding affinity to recombinant human full-length N-terminal His8-tagged Wee1 (1 to 646 residues) expressed in human Expi293F cells assessed as desso...More data for this Ligand-Target Pair
TargetMembrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase(Human)
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Affinity DataKd: 324nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged Myt1 kinase domain (75 to 361 residues) by isothermal titration calorimetryMore data for this Ligand-Target Pair
Affinity DataKd: 13nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged Wee1 kinase domain (291 to 575 residues) expressed in Escherichia coli BL21 (DE3) by iso...More data for this Ligand-Target Pair
Affinity DataIC50: 5.20nMAssay Description:Inhibition of recombinant human Wee1 using poly(Lys,Tyr) as substrate after 30 mins in presence of [gamma-33P]-ATP by liquid scintillation counting m...More data for this Ligand-Target Pair
TargetMembrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase(Human)
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Affinity DataIC50: 4.94E+3nMAssay Description:Inhibition of human full length PKMYT1 expressed in HEK293 cells using EFS (247 to 259 residues) as substrate after 1 hr by fluorescence polarization...More data for this Ligand-Target Pair
Affinity DataIC50: 350nMAssay Description:Inhibition of PLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 2.31E+3nMAssay Description:Inhibition of human WNK3 using MBP as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 101nMAssay Description:Inhibition of human WEE1 using MBP as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 5.20nMAssay Description:Inhibition of N-terminal GST-fused human WEE1 (215 to 646 residues) expressed in baculovirus expression system using poly(Lys,Tyr) as substrate incub...More data for this Ligand-Target Pair
TargetMembrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase(Human)
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Affinity DataKi: 470nMAssay Description:Inhibition of human full length PKMYT1 expressed in HEK293 cells using EFS (247 to 259 residues) as substrate after 1 hr by fluorescence polarization...More data for this Ligand-Target Pair
TargetMembrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase(Human)
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Martin-Luther-University Halle-Wittenberg
Curated by ChEMBL
Affinity DataKi: 601nMAssay Description:Inhibition of N-[3',6'-dihydroxy-3-oxo-3H-spiro(2-benzofuran-1,9'-xanthen)-5-yl]-N'-[2-(4-{4-[N-(2-chloro-6-methylphenyl)-5-carboxamido]-thiazol-2-yl...More data for this Ligand-Target Pair